interleukin-17 gene expression and serum levels in acute rejected and non-rejected liver transplant patients

Authors

afsoon afshari

department of molecular genetics, science and research, islamic azad university ramin yaghobi

shiraz transplant research center, shiraz university of medical sciences, shiraz, iran mohammad hossein karimi

shiraz transplant research center, shiraz university of medical sciences, shiraz, iran mojtaba darbooie

department of molecular genetics, science and research, islamic azad university negar azarpira

abstract

background: interleukin-17 (il-17), as a potent proinflammatory cytokine, has a critical role in post liver transplant outcomes. however, there is not much information about the effects of il-17 cytokine on acute liver rejection. objective: to evaluate the role of il-17 in post-liver transplant acute rejection. methods: ninety seven adult liver transplant patients who enrolled in this cross sectional study were divided into non- acute rejected (non-ar) and acute rejected (ar) patient groups. three blood samples were collected from each patient in days 1, 4 and 7 post liver transplantation. the il-17 mrna levels were evaluated using an in-house real time pcr protocol. il- 17 protein levels were also analyzed in non-ar, ar and also control groups using elisa method. results: the il-17 mrna expression level continuously increased in ar patients in all days of follow-up post liver transplantation. il-17 expression was, however, down regulated after day 4 post-transplant follow-up in non-ar patients. both il-17 mrna expression and protein levels were also significantly increased in ar patients compared with non-ar ones. conclusion: based on these findings, significant increase of il-17 mrna and protein levels in ar patients highlights the important role of il-17 in acute liver rejection.

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Journal title:
iranian journal of immunology

جلد ۱۱، شماره ۱، صفحات ۲۹-۳۹

Keywords
[ ' a c u t e r e j e c t i o n ' , ' i n t e r l u k i n ' , 1 7 , ' l i v e r t r a n s p l a n t a t i o n ' ]

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